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مقاله
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Abstract
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Title:
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In-vitro and In-vivo treatment of fungal Keratitis by daily treatment of cold atmospheric pressure plasma jet (CAPPJ)
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Author(s):
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Hamed Nikmaram 1, Maryam Amini 1, Mozhgan Rezaei Kanavi 2, Mahmood Ghoranneviss 1, Sahar Balagholi 2
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Presentation Type:
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Oral
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Subject:
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Cornea
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Others:
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Presenting Author:
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Name:
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Hamed Nikmaram
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Affiliation :(optional)
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1-Plasma Physics Research Center,Science and Research Branch of Islamic Azad university,Tehran,Iran. 2-Ocular Tissue Engineering Research Center,Shahid Beheshti University of Medical Sciences,Tehran,Ir
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E mail:
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nikmaram@gmail.com
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Phone:
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09199350112
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Mobile:
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09199350112
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Purpose:
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To investigate in-vitro and in-vivo treatment of fungal keratitis by helium cold atmospheric pressure plasma jet (CAPPJ).
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Methods:
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Suspensions of Aspergilous fumigatus with 10 6 colony forming units were exposed to CAPPJ 5 minutes daily for 7 days. After culturing the post-treatment suspension in Saboraud agar, fungal colonies were counted. As for in vivo study, fungal keratitis with Aspergilous fumigatus was induced in 3 rabbits and subsequently was treated with CAPPJ 5 minutes daily for 7 days. Size of keratitis was daily evaluated daily and after 7 days microbiologic and histopathologic investigations were performed.
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Results:
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The results obtained from in vitro treatment of Aspergillus fumigatus proved the efficacy of CAPPJ to inactivate fungal microorganisms. Direct application of the plasma jet in experimental model of fungal keratitis resulted in significant reduction of the size of keratitis; the clinical results were supported by the microbiologic and histopathologic results.
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Conclusion:
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Both in vitro and in vivo results showed that CAPPJ treatment was effective in inactivating fungal pathogens and therefore it may be considered as a new and safe treatment modality for fungal keratitis.
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Attachment:
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