The tissue engineering aims to design and engineering the structures to regeneration and repair of damaged tissues in body. Polymeric scaffolds are the most important material and a requirement of these designs. The technology engineering of this design by using the polymeric scaffolds (polymeric smart substrate and Nano fibers) tries to design sufficient cell scaffold for Limbal stem cell of eyes.

This study has designed a sufficient substrate for engineering the cell sheet by grafting the sensitive to temperature smart polymer via UV radiation on poly styrene pane. This study has proved the structural analysis ATR-FTIR in presence of Acryl amid groups grafted to poly styrene surface , DSC analysis , polymerization process and the presence of smart polymer because of phase change from liquid to solid and also the sample contact angle represents the relationship between surface rough and wetting of graft surfaces with smart polymer .the cell investigations with culture of epithelial cells on smart surfaces represent that cells will be cultured well on grafting substrate in physiological temperature (370C) and the cell sheets will be detached from surface because of hydrophobic characteristic

Separation and culturing of human USSC cells was done from Umbilical cord blood. These cells were cultured to 50 passages continuously. . The multiplication and morphology of these cells before freezing and after thawing were same .During different stages of operation, there were not observed any sign of contamination of these cells to viruses or mycoplasmas. Since USSC cells must be operated as a supportive layer in long time, some of the primary passages were freezed to provide a sufficient storage of these cells. USSC cells are spindle shape morphologically with the size equal to 20-25 µm.

In sum, the nano fiber sample has shown good results as a sufficient scaffold to regenerate cornea epithelial tissue, so these samples can be used to study the animal samples.