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مقاله
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Abstract
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Title:
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Subretinal transplantation of functional RPE derived from bone marrow stromal stem cells improved visual function in rat model for RPE degeneration
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Author(s):
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Hamid Aboutaleb kadkhodaeian , Taki Tiraihi,, Hamid Ahmadieh , Hosein ziaii Ardakani , Narsis Daftarian .
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Presentation Type:
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Oral
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Subject:
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Retina and Retinal Cell Biology
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Others:
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Presenting Author:
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Name:
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Hamid Aboutaleb kadkhodaeean
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Affiliation :(optional)
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1Dept. of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14155-4838, Tehran, Iran. 2Department of Ophthalmology Labbafinejad Medical Center Pasdaran Ave. Boostan
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E mail:
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habootaleb92@gmail.com
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Phone:
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Mobile:
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09377711768
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Purpose:
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To differentiate the bone marrow stromal stem cells (BMSCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases.
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Methods:
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bone marrow stromal stem cells extract from bone marrow of pigmented rats and after reaching to passage 3-4, induce to neural stem cells and finally differentiated to functional RPE cells. The nuclei of these cells labeled with BrdU and then transplanted to subretinal space of RPE injury animal model induced with sodium iodate. 7, 14, 30, 60 and 90 days post transplantation, ERG recorded and immunohistochemistry and crysel violet staining performed on sections from transplantation site.
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Results:
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result of ERG showed that 90 days after transplantation, a-wave and b-wave improved when compared with control group. And also immunohistochemistry of sections showed that transplanted RPE migrate and integrated to host RPE layer. The morphometry of sections reveal that the number of nucleus in ONL increased when compared with control group.
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Conclusion:
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in this study we showed that transplantation of functional derived RPE from BMSCs could migrate and integrated to host retina damaged with sodium iodate and restore the function of retina.
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Attachment:
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