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       XXII Annual Congress of the Iranian Society of Ophthalmology        بـیــست و دومــیــن کنــگــره سـالیـانه انـجـمـن چـشـم پـزشـکی ایـــران
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مقاله Abstract


Title: Investigating the Effect of PAX6 Gene Expression on Differentiation of CD133+Human Cord Blood Stem Cells
Author(s): Balagholi, Sahar1, 2; Amini Kafiabadi, Sedigheh1 ; Soheili, Zahra-Soheila3 ; Rezaeikanavi, Mozhgan2, 4; Samiei, Shahram
Presentation Type: Poster
Subject: Molecular Biology and Genetics
Others:
Presenting Author:
Name: Sahar Balagholi
Affiliation :(optional) 1.Iranian Blood Transfusion Organization, Tehran, Iran (the Islamic Republic of).2. Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
E mail: sahar_balagholi@yahoo.com
Phone: 02122592200
Mobile: 09367124753
Purpose:

To assess the influence of PAX6 gene expression on differentiation of CD133+ human cord blood stem cells

Methods:

Human cord blood mononuclear cells were isolated by ficoll. CD133+ cells were obtained using the CD133MicroBead Kit in combination with the autoMACS Separator and then cultured in the Stem Span media. HEK293T packaging cells were co-transfected with PLEX-MCS, PsPAX2, and pMD2G via calcium phosphate method. The resultant lentiviral vectors were collected and concentrated by PEG 6000. The appropriate amount of viruses was used for infecting CD133+ cells. The successful transduced cells were selected by puromycin resistance. After two weeks, the expression of rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins were assayed by immunocytochemistry.

Results:

Two weeks after transfection, CD133+ cells expressed rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins, as compared to the HEK293T cells that expressed rhodopsin and nestin.

Conclusion:

Transfected CD133+ human cord blood stem cells with PAX6 gene are able to differentiate into progenitor retinal neural- and ganglion-like cells.

Attachment: 2031sahar balagholi.pptx





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  - بـیــست و دومــیــن کنــگــره سـالیـانه انـجـمـن چـشـم پـزشـکی ایـــران