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مقاله
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Abstract
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Title:
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Investigating the Effect of PAX6 Gene Expression on Differentiation of CD133+Human Cord Blood Stem Cells
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Author(s):
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Balagholi, Sahar1, 2; Amini Kafiabadi, Sedigheh1 ; Soheili, Zahra-Soheila3 ; Rezaeikanavi, Mozhgan2, 4; Samiei, Shahram
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Presentation Type:
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Poster
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Subject:
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Molecular Biology and Genetics
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Others:
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Presenting Author:
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Name:
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Sahar Balagholi
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Affiliation :(optional)
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1.Iranian Blood Transfusion Organization, Tehran, Iran (the Islamic Republic of).2. Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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E mail:
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sahar_balagholi@yahoo.com
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Phone:
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02122592200
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Mobile:
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09367124753
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Purpose:
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To assess the influence of PAX6 gene expression on differentiation of CD133+ human cord blood stem
cells
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Methods:
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Human cord blood mononuclear cells were isolated by ficoll. CD133+ cells were obtained using the
CD133MicroBead Kit in combination with the autoMACS Separator and then cultured in the Stem Span media.
HEK293T packaging cells were co-transfected with PLEX-MCS, PsPAX2, and pMD2G via calcium phosphate method.
The resultant lentiviral vectors were collected and concentrated by PEG 6000. The appropriate amount of viruses was
used for infecting CD133+ cells. The successful transduced cells were selected by puromycin resistance. After two
weeks, the expression of rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins were assayed by
immunocytochemistry.
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Results:
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Two weeks after transfection, CD133+ cells expressed rhodopsin, CHX10, Thy1, nestin, and PAX6 proteins,
as compared to the HEK293T cells that expressed rhodopsin and nestin.
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Conclusion:
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Transfected CD133+ human cord blood stem cells with PAX6 gene are able to differentiate into
progenitor retinal neural- and ganglion-like cells.
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Attachment:
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2031sahar balagholi.pptx
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